MDMA (Ecstasy) decreases the number of neurons and stem cells in embryonic cortical cultures.
Anna M S Kindlundh-Högberg, Chris Pickering, Grzegorz Wicher, David Hobér, Helgi B Schiöth, Asa Fex Svenningsen
Cellular and molecular neurobiology January 2010 DOI: 10.1007/s10571-009-9426-y (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory study Peer reviewed |
|---|---|
| Population | Dissociated CNS cortex cells from rat embryos (E17) |
| Intervention | MDMA |
| Dose | 100, 200, and 400 microM |
| Duration | 5 days after single exposure |
| Measures | nestin-positive cell density, NeuN-positive cell density, qPCR mRNA expression |
| Topics | MDMA |
| Key points | MDMA caused a dose-dependent decrease in nestin-positive stem cells and reduced NeuN-positive neurons at 400 micromolar. It also altered mRNA expression of neurotransmitter receptors and transporters, indicating potential neurodevelopmental toxicity. |
Abstract
Ecstasy, 3,4-methylenedioxymetamphetamine (MDMA), is a recreational drug used among adolescents, including young pregnant women. MDMA passes the placental barrier and may therefore influence fetal development. The aim was to investigate the direct effect of MDMA on cortical cells using dissociated CNS cortex of rat embryos, E17. The primary culture was exposed to a single dose of MDMA and collected 5 days later. MDMA caused a dramatic, dose-dependent (100 and 400 microM) decrease in nestin-positive stem cell density, as well as a significant reduction (400 microM) in NeuN-positive cells. By qPCR, MDMA (200 microM) caused a significant decrease in mRNA expression of the 5HT3 receptor, dopamine D(1) receptor, and glutamate transporter EAAT2-1, as well as an increase in mRNA levels of the NMDA NR1 receptor subunit and the 5HT(1A) receptor. In conclusion, MDMA caused a marked reduction in stem cells and neurons in embryonic cortical primary cell cultures, which was accompanied by changes in mRNA expression of specific receptors and transporters for glutamatergic and monoaminergic neurotransmitters.