The mixture of "ecstasy" and its metabolites impairs mitochondrial fusion/fission equilibrium and trafficking in hippocampal neurons, at in vivo relevant concentrations.
Daniel José Barbosa, Roman Serrat, Serena Mirra, Martí Quevedo, Elena Goméz De Barreda, Jesús Àvila, Luísa Maria Ferreira, Paula Sério Branco, Eduarda Fernandes, Maria de Lourdes Bastos, João Paulo Capela, Eduardo Soriano, Félix Carvalho
Toxicological sciences : an official journal of the Society of Toxicology June 2014 DOI: 10.1093/toxsci/kfu042 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro experimental study Peer reviewed |
|---|---|
| Population | Cultured hippocampal neurons |
| Dose | 10 μM each compound |
| Topics | MDMA |
| Keywords | 3,4-Methylenedioxymethamphetamine MDMA MDMA'S Metabolites Mitochondrial dysfunction Mitochondrial fusion/fission Mitochondrial trafficking Neurotoxicity |
| Key findings | A mixture of MDMA and six of its major in vivo metabolites, each at 10 μM, impaired mitochondrial trafficking and increased axonal mitochondrial fragmentation in cultured hippocampal neurons. Overexpression of Mfn2 or Drp1 K38A almost completely rescued the trafficking deficits, and the Mfn2 R94Q mutant confirmed that dysregulated fission/fusion contributes to the phenotype. The authors propose that disrupted mitochondrial dynamics may underlie MDMA-related neurotoxicity. |
Abstract
3,4-Methylenedioxymethamphetamine (MDMA; "ecstasy") is a potentially neurotoxic recreational drug of abuse. Though the mechanisms involved are still not completely understood, formation of reactive metabolites and mitochondrial dysfunction contribute to MDMA-related neurotoxicity. Neuronal mitochondrial trafficking, and their targeting to synapses, is essential for proper neuronal function and survival, rendering neurons particularly vulnerable to mitochondrial dysfunction. Indeed, MDMA-associated disruption of Ca(2+) homeostasis and ATP depletion have been described in neurons, thus suggesting possible MDMA interference on mitochondrial dynamics. In this study, we performed real-time functional experiments of mitochondrial trafficking to explore the role of in situ mitochondrial dysfunction in MDMA's neurotoxic actions. We show that the mixture of MDMA and six of its major in vivo metabolites, each compound at 10μM, impaired mitochondrial trafficking and increased the fragmentation of axonal mitochondria in cultured hippocampal neurons. Furthermore, the overexpression of mitofusin 2 (Mfn2) or dynamin-related protein 1 (Drp1) K38A constructs almost completely rescued the trafficking deficits caused by this mixture. Finally, in hippocampal neurons overexpressing a Mfn2 mutant, Mfn2 R94Q, with impaired fusion and transport properties, it was confirmed that a dysregulation of mitochondrial fission/fusion events greatly contributed to the reported trafficking phenotype. In conclusion, our study demonstrated, for the first time, that the mixture of MDMA and its metabolites, at concentrations relevant to the in vivo scenario, impaired mitochondrial trafficking and increased mitochondrial fragmentation in hippocampal neurons, thus providing a new insight in the context of "ecstasy"-induced neuronal injury.
Comparable studies
Other preclinical and animal studies on MDMA, most cited first.
Citations in the library
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- Lysosomal Dysfunction and Autophagy Blockade Contribute to MDMA-Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells. Chemical Research in Toxicology April 20, 2020
- Of mice and men on MDMA: a translational comparison of the neuropsychobiological effects of 3,4-methylenedioxymethamphetamine ('Ecstasy'). Brain Research November 14, 2019
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