A harm-reduction approach to the isolation of harmine and its hydrogenated derivatives from Peganum Harmala seed in low-tech settings
Frederick A.P. van der Sypt
Zenodo (CERN European Organization for Nuclear Research) April 10, 2019 preprint DOI: 10.5281/zenodo.4660024 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Practical guide or manual |
|---|---|
| Intervention | zinc-acetic acid reduction |
| Measures | microscopy, melting point determination |
| Key points | The authors report that harmine and harmaline can be separated from Peganum harmala seed extract by pH-metry with 91% recovery or by sodium bicarbonate precipitation with 76% yield, and that zinc-acetic acid reduces harmaline to tetrahydroharmine at 83% yield, though harmine resists this hydrogenation. They propose these protocols as harm-reduction tools for low-tech settings. |
Abstract
ABSTRACT Background. Peganum Harmala (Syrian rue, Nitrariaceae) has a long and widespread history of ethnopharmacological use. Due to its rich and diverse alkaloid content, the traditional application of whole plant material or full alkaloid extract is inherently unspecific and therefore susceptible to side effects. Simultaneously, incorrect phytochemical procedures for its use in ayahuasca-analogues cause intoxications in misinformed users. Aim of the study. Providing harm-reduction by developing easily applicable, safe and effective isolation protocols for harmine, harmaline (dihydroharmine) and tetrahydroharmine (leptaflorine) from Peganum Harmala seed. Materials and methods. Only commonly available equipment and reagents were used for isolating the alkaloids. Following extraction, harmine and harmaline were separated using 2 low-tech methods (selective precipitation using sodium bicarbonate or pH-metry). Then, zinc-acetic acid reduction of both harmine and harmaline was attempted. The identity and purity of the obtained alkaloids were confirmed using microscopy and melting point determination. Results. Using pH-metry for the separation of harmine and harmaline proved rapid, effective and recovered 91% of alkaloids. Selective precipitation of the alkaloids with sodium bicarbonate had a yield of 76%. Hydrogenation of harmaline to tetrahydroharmine using zinc-acetic acid had a yield of 83%. Harmine however could not be hydrogenated by zinc-acetic acid. The melting ranges of the obtained alkaloids were narrow and consistent with literature. Based on these results, isolation protocols were developed. Conclusions. Harmine, harmaline and tetrahydroharmine can be isolated with good yields from Peganum Harmala seed following protocols that are applicable in traditional and other low-tech settings. This study showed zinc-acetic acid to be effective in the reductive synthesis of tetrahydroharmine and also proposes a rapid, precise and high yielding method for the bulk separation of harmine and harmaline using basic pH-metry.