D-lysergic acid reduces microtubule-associated tau protein in SH-SY5Y human neuroblastoma cells.
General pharmacology September 1995 DOI: 10.1016/0306-3623(94)00272-o (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro cell study Peer reviewed |
|---|---|
| Population | SH-SY5Y human neuroblastoma cell line |
| Intervention | LSD |
| Dose | 10(-5) M and 10(-7) M |
| Duration | 48 hours |
| Key points | LSD treatment for 48 hours reduced 50 kDa tau protein in the membrane fraction of SH-SY5Y cells at 10(-5) and 10(-7) M, and reduced tau in the cytoplasmic fraction at 10(-5) M. At 10(-7) M, total cytoplasmic protein increased by 65% (P < 0.05). The authors hypothesize that these changes in tau metabolism may affect microtubule assembly and neurotransmitter transport. |
Abstract
1. SH-SY5Y, an adrenergic human neuroblastoma cell line, was used to examine the hypothesis that D-lysergic acid (LSD) affects the metabolism of microtubule-associated tau protein, thus affecting microtubule assembly and the transport of neurotransmitters. 2. After 48 hr treatment LSD (10(-5) and 10(-7) M) decreased 50 kDa tau protein in the membrane (pellet) fraction. The drug (10(-5) M) also decreased in the cytoplasmic (supernatant) fraction. 3. This reduction in tau protein was accompanied by a 65% increase (P < 0.05) in total protein after LSD (10(-7) M) in the cytoplasmic fraction.