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A fatal case of poisoning related to new cathinone designer drugs, 4-methoxy PV8, PV9, and 4-methoxy PV9, and a dissociative agent, diphenidine.

Keiko Kudo, Yosuke Usumoto, Ruri Kikura-Hanajiri, Naomi Sameshima, Akiko Tsuji, Noriaki Ikeda

Legal medicine (Tokyo, Japan) September 1, 2015 DOI: 10.1016/j.legalmed.2015.06.005 (opens in new tab) via PubMed

Summary

AI-generated from the abstract

A woman in her thirties was found dead with aroma liquid, bath salt products, and hypnotic drug tablets nearby. Autopsy revealed pulmonary congestion and edema. Analysis detected four designer drugs: 4-methoxy PV8, PV9, 4-methoxy PV9, and diphenidine, plus three benzodiazepines (triazolam, flunitrazepam, nitrazepam) and alcohol. Femoral blood concentrations of the cathinones and diphenidine were 2.69, 0.743, 0.261, and 1.38 μg/ml, respectively, markedly higher than earlier cases; alcohol was 1.52 mg/ml. The cause of death was poisoning from these cathinone drugs and diphenidine, compounded by benzodiazepines and alcohol.

Study at a glance

Characteristics Case study Case report Peer reviewed
Sample size 1
Population A woman in her thirties
Keywords Diphenidine Gc–ms Lc–ms/ms Pv9 α-pop
Key finding Death resulted from poisoning by three cathinone designer drugs and diphenidine, with contributing benzodiazepines and alcohol.

Abstract

A woman in her thirties was found dead on a bed. Considerable amounts of "aroma liquid" and "bath salt" products and hypnotic drug tablets were scattered beside the bed. Autopsy showed pulmonary congestion and edema. Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analyses of "aroma liquid" and "bath salt" products showed the presence of new cathinone designer drugs, 4-methoxy PV8 (4-methoxy PHPP), PV9 (α-POP), and 4-methoxy PV9 (4-methoxy α-POP), and a dissociative agent, diphenidine. Drug screening in stomach contents, blood and hydrolyzed urine of the woman by GC-MS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed the presence of the above 4 types of drugs and 3 types of benzodiazepines, triazolam, flunitrazepam, and nitrazepam, and their metabolites. The above 7 drugs and 3 benzodiazepine metabolites were simultaneously determined by LC-MS/MS after modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction using diazepam-d5 as the internal standard. The concentrations of 4-methoxy PV8, PV9, 4-methoxy PV9, and diphenidine in the femoral blood were 2.69, 0.743, 0.261, and 1.38μg/ml, respectively, which were significantly higher than concentrations reported in previous cases. Alcohol concentration in the femoral blood was 1.52mg/ml. Based on the pathological and toxicological findings, the cause of death was determined to be 3 types of cathinone drugs, 4-methoxy PV8, PV9, and 4-methoxy PV9, and diphenidine poisoning under the influence of 3 benzodiazepines and alcohol.

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