Electroencephalographic Spectral and Coherence Analysis of Ketamine in Rats: Correlation with Behavioral Effects and Pharmacokinetics
Tomáš Páleníček, Michaela Fujáková, Martin Brunovský, Marie Balı́ková, Jiřı́ Horáček, Ingmar Gorman, Filip Tylš, B. Tislerova, Peter Šóš, Vĕra Bubeníková‐valešová, Cyril Höschl, Vladimı́r Krajča
Neuropsychobiology 2011 DOI: 10.1159/000321803 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Rats |
| Intervention | Ketamine |
| Dose | 9 and 30 mg/kg (i.p.) |
| Topics | Ketamine Esketamine |
| Keywords | Electroencephalography Prepulse inhibition Pharmacokinetics Startle response Pharmacology Anesthesia Schizophrenia object-oriented programming |
| Citations | 85 |
| Key findings | Ketamine at behaviorally active doses induces a robust increase in EEG power spectra and coherence, with maximum changes correlating with the kinetics of the drug. |
Abstract
Aims: This study was designed to evaluate the changes in EEG power spectra and EEG coherence in a ketamine model of psychosis in rats. Analyses of behavioral measurements--locomotion and sensorimotor gating--and the pharmacokinetics of ketamine and norketamine were also conducted.
Methods: Ketamine and norketamine levels in rat sera and brains were analyzed by gas chromatography-mass spectrometry after ketamine 30 mg/kg (i.p.). Ketamine 9 and 30 mg/kg (i.p.) were used in the behavioral and EEG experiments. Locomotor effects in an open field test and deficits in prepulse inhibition of acoustic startle reaction (PPI ASR) were evaluated in the behavioral experiments. EEG signals were simultaneously recorded from 12 implanted active electrodes; subsequently, an EEG power spectral and coherence analysis was performed.
Results: Ketamine had a rapid penetration into the brain; the peak concentrations of the drug were reached within 15 min after administration. Ketamine induced marked hyperlocomotion and deficits in the PPI ASR. EEG spectral analysis mainly showed increases in EEG power as well as coherence. These were most robust at 10-15 min after the administration and influenced all parts of the spectrum with ketamine 30 mg/kg.
Conclusions: Ketamine at behaviorally active doses induces a robust increase in EEG power spectra and coherence. The maximum levels of change correlated with the kinetics of ketamine.