Proliferative Effects of the Psychedelic N,N-Dimethyltryptamine (DMT) in Human Neural Stem Cells.
José Alexandre Salerno, Elizabeth R. Dominguez, Karina Karmirian, Breno A.b.m.s. Arrais, Juliano Alves, Giovanna Erjautz, Kennedy Kroening, Leticia R. Q. Souza, Isis M. Ornelas, Stevens K. Rehen
ACS Chemical Neuroscience July 9, 2026 DOI: 10.1021/acschemneuro.6c00209 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro experimental study Peer reviewed |
|---|---|
| Population | Human iPSC-derived neural stem cells |
| Intervention | DMT |
| Dose | concentration-dependent, half-maximal effect at 59.7 nM |
| Duration | 24 h treatment; washout followed by neurosphere formation up to day 10 |
| Topics | 5-MeO-DMT DMT |
| Keywords | Bdnf N,N-Dimethyltryptamine DMT Cell cycle Neurosphere |
| Key findings | 24-hour DMT treatment increased proliferation of human neural stem cells in a concentration-dependent manner and upregulated G1 cell-cycle regulators and BDNF expression. |
Abstract
The serotonergic psychedelic N,N-dimethyltryptamine (DMT) produces rapid antidepressant effects in preclinical and early clinical studies. Therapeutic benefits have been linked to sustained neural plasticity, including adult neurogenesis in rodents. Whether brief DMT exposure engages proliferative responses in human neural stem cells (NSCs) remains unresolved. Using human iPSC-derived NSCs, we found that 24 h DMT treatment increased proliferation in a concentration-dependent manner (half-maximal effect at 59.7 nM) and upregulated G1 cell-cycle regulators. DMT also shifted trophic gene expression, decreasing neurotrophin-3 while increasing nerve growth factor and brain-derived neurotrophic factor (BDNF) transcripts and intracellular BDNF protein. After washout, DMT-primed NSCs formed larger neurospheres, with progenitor and early neuronal marker composition matching controls by day 10. These findings demonstrate that brief DMT exposure is sufficient to engage proliferative and neurotrophin-associated responses in human NSCs at concentrations consistent with those reported for DMT-induced plasticity across other systems.
Comparable studies
Other preclinical and animal studies on 5-MeO-DMT, most cited first.
| Study | Year | Design | Participants |
|---|---|---|---|
| Psychedelic N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine modulate innate and adaptive inflammatory responses through the sigma-1 receptor of human monocyte-derived dendritic cells. Human primary monocyte-derived dendritic cells | 2014 | In vitro study | |
| Effects of N,N-Dimethyltryptamine on Rat Behaviors Relevant to Anxiety and Depression Adult male Sprague-Dawley rats | 2018 | Experimental study | |
| Hallucinogen-like actions of 5-methoxy-N,N-diisopropyltryptamine in mice and rats. Mice and rats | 2006 | Preclinical study | |
| Agonist Properties of N,N-Dimethyltryptamine at Serotonin 5-HT2A and 5-HT2C Receptors Rats and transfected fibroblasts | 1998 | Experimental study | |
| Subtypes of the 5-HT receptor mediating the behavioural responses to 5-methoxy-N,N-dimethyltryptamine in the rat Intact rats and rats depleted of monoamines with reserpine | 1985 | Experimental study |
Citations in the library
Cites 21
- Psychedelics Promote Structural and Functional Neural Plasticity Cell Reports June 1, 2018
- Rapid antidepressant effects of the psychedelic ayahuasca in treatment-resistant depression: a randomized placebo-controlled trial Psychological Medicine June 15, 2018
- Psychedelics promote neuroplasticity through the activation of intracellular 5-HT2A receptors Science February 16, 2023
- Correlation between the potency of hallucinogens in the mouse head-twitch response assay and their behavioral and subjective effects in other species. Neuropharmacology May 1, 2020
- Effects of psilocybin on hippocampal neurogenesis and extinction of trace fear conditioning Experimental Brain Research June 1, 2013
and 16 more in the library