Investigating the psychedelic hypothesis of kykeon, the sacred elixir of the Eleusinian Mysteries
Romanos Konstantinos Antonopoulos, Evangelos Dadiotis, Kostas Ioannidis, Antigoni Cheilari, Vangelis Mitsis, Ana M. Garcia-Campaña, Laura Gámiz-gracia, Maykel Hernández-mesa, Alfonso Narváez, Mark Anthony Hoffman, Carl A. P. Ruck, Zacharoula Gonou‐zagou, Aligiannis Nektarios, Prokopios Magiatis
Scientific Reports February 13, 2026 DOI: 10.1038/s41598-026-39568-3 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental study Peer reviewed |
|---|---|
| Duration | 120 minutes |
| Keywords | Lysergic acid Amide Elixir programming language Claviceps purpurea Transformation genetics Stereochemistry Acid hydrolysis |
| Key findings | Boiling ergot in lye converts toxic ergopeptides into psychoactive lysergic acid amide and isolysergic acid amide, supporting the psychedelic Eleusis hypothesis. |
Abstract
This study revisits the hypothesis that Claviceps purpurea (Fr.) Tul., a fungus infecting cereals and producing ergot alkaloids (EAs), was the psychedelic agent in kykeon, the sacred elixir of the Eleusinian Mysteries. Although archaeological evidence supports this link, experimental data confirming the transformation of toxic ergopeptides into psychoactive lysergic acid amide derivatives in putative ancient preparations remain limited. The potential hydrolysis products of ergot were investigated using a technique that could have been readily applied by the priestesses of Eleusis, notably reacting in lye. Pulverized sclerotia were refluxed in solutions of lye and in distilled water. Samples obtained after liquid-liquid extractions were analyzed with 1H-NMR and UHPLC/Q-TOF-HRMS. The 1H NMR spectra demonstrated conversion of toxic ergopeptides, present in control samples but absent at all reaction times in 5% w/v ergot treated with pH 12.5 lye, where characteristic peaks for lysergic acid amide (LSA; ergine) and isolysergic acid amide (iso-LSA; isoergine; erginine) appeared. UHPLC/Q-TOF-HRMS quantitation confirmed this conversion, yielding 0.54 mg LSA and 0.48 mg iso-LSA per gram of ergot at 120 min. The chemical transformation of toxic EAs to psychoactive compounds, utilizing ancient technology, was demonstrated and supports the “psychedelic Eleusis” hypothesis.