Analysis of Serotonin in Caenorhabditis Elegans Subjected to Micro-Dosing with Psilocybin| Iris Publishers
Douglas B. Craig
Insights in Chemistry and Biochemistry October 15, 2025 DOI: 10.33552/icbc.2025.03.000562 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Caenorhabditis elegans |
| Intervention | psilocybin |
| Topics | Serotonin Microdosing Psilocybin |
| Keywords | Caenorhabditis elegans Iris biosensor Capillary electrophoresis Fluorescence Molecular biology Serotonin antagonists Chromatography Extraction chemistry |
| Key findings | Psilocybin decreased serotonin concentration in C. elegans from 0.9 to 0.5 mg/mg protein. |
Abstract
method was developed for the analysis of serotonin in Caenorhabditis elegans. Samples were subjected to solvent-solvent extraction from basic conditions into n-heptanol. The serotonin was then back-extracted into acetic acid, the solution diluted into basic buffer, and labelled with 3-(2-furoyl) quinoline-2-carboxaldehyde in the presence of cyanide. The sample was analyzed by capillary electrophoresis utilizing post-column laser-induced fluorescence detection within a sheath flow cuvette. Under these conditions serotonin was detected at 1 M. C. elegans was cultured in the presence and absence of psilocybin. The presence of psilocybin was found to decrease the concentration of serotonin from 0.9 to 0.5 mg/mg protein.
Comparable studies
Other experimental studies on microdosing, most cited first.