Automated Extraction of Lysergic Acid Diethylamide (LSD) and N-demethyl-LSD from Blood, Serum, Plasma, and Urine Samples Using the Zymark RapidTrace™ with LC/MS/MS Confirmation
John de Kanel, W E Vickery, B Waldner, R M Monahan, F X Diamond
Journal of Forensic Sciences May 1, 1998 DOI: 10.1520/jfs16192j (opens in new tab) via OpenAlex
Summary
AI-generated from the abstractA fully automated solid-phase extraction method using the Zymark RapidTrace™ followed by liquid chromatography and tandem mass spectrometry (LC-MS/MS) can quantify lysergic acid diethylamide (LSD) and confirm its metabolite N-demethyl-LSD in blood, serum, plasma, and urine. LSD quantitation uses an internal standard (LSD-d3). The limit of quantitation for LSD is 0.05 ng/mL, and the limit of detection for both LSD and its metabolite is 0.025 ng/mL. Recovery of LSD exceeds 95% at 0.1 and 2.0 ng/mL. At 1.0 ng/mL LSD, within-run relative standard deviation is 2.2% and between-run is 4.4%.
Study at a glance
| Characteristics | Method development and validation Qualitative Peer reviewed |
|---|---|
| Topics | LSD |
| Keywords | Urine Chromatography Extraction chemistry Biochemistry |
| Citations | 22 |
| Key finding | The automated extraction and LC-MS/MS method reliably quantifies LSD down to 0.05 ng/mL and detects its metabolite at 0.025 ng/mL in biological fluids with high recovery and precision. |
Abstract
Abstract A forensic procedure for the quantitative confirmation of lysergic acid diethylamide (LSD) and the qualitative confirmation of its metabolite, N-demethyl-LSD, in blood, serum, plasma, and urine samples is presented. The Zymark RapidTrace™ was used to perform fully automated solid-phase extractions of all specimen types. After extract evaporation, confirmations were performed using liquid chromatography (LC) followed by positive electrospray ionization (ESI+) mass spectrometry/mass spectrometry (MS/MS) without derivatization. Quantitation of LSD was accomplished using LSD-d3 as an internal standard. The limit of quantitation (LOQ) for LSD was 0.05 ng/mL. The limit of detection (LOD) for both LSD and N-demethyl-LSD was 0.025 ng/mL. The recovery of LSD was greater than 95% at levels of 0.1 ng/mL and 2.0 ng/mL. For LSD at 1.0 ng/mL, the within-run and between-run (different day) relative standard deviation (RSD) was 2.2% and 4.4%, respectively.