LC-MS Analysis of Human Urine Specimens for 2-Oxo-3-Hydroxy LSD: Method Validation for Potential Interferants and Stability Study of 2-Oxo-3-Hydroxy LSD Under Various Storage Conditions
K. L. Klette, C. K. Horn, Peter Stout, Carolyn J. Anderson
Journal of Analytical Toxicology May 1, 2002 DOI: 10.1093/jat/26.4.193 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory study Peer reviewed |
|---|---|
| Duration | 0 to 9 days for temperature, pH, and light exposure; 0 to 60 days for long-term frozen storage |
| Topics | LSD |
| Keywords | Urine Metabolite Hallucinogen Liquid chromatography–mass spectrometry Drugs of abuse |
| Citations | 39 |
| Key points | No compounds tested interfered with detection of O-H-LSD, and the metabolite was stable under refrigerated and frozen conditions within normal urine pH, but degraded at room temperature or higher. |
Abstract
2-Oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD), a major LSD metabolite, has previously been demonstrated to be a superior marker for identifying LSD use compared with the parent drug, LSD. Specifically, O-H-LSD analyzed using liquid chromatography-mass spectrometry has been reported to be present in urine at concentrations 16 to 43 times greater than LSD. To further support forensic application of this procedure, the specificity of the assay was assessed using compounds that have structural and chemical properties similar to O-H-LSD, common over-the-counter products, prescription drugs and some of their metabolites, and other drugs of abuse. Of the wide range of compounds studied, none were found to interfere with the detection of O-H-LSD or the internal standard 2-oxo-3-hydroxy lysergic acid methyl propylamide. The stability of O-H-LSD was investigated from 0 to 9 days at various temperatures, pH conditions, and exposures to fluorescent light. Additionally, the effect of long-term frozen storage and pH was investigated from 0 to 60 days. There was no significant loss of O-H-LSD under both refrigerated and frozen conditions within the normal human physiological pH range of urine (4.6-8.4). However, significant loss of O-H-LSD was observed in samples prepared at pH 4.6-8.4 and stored at room temperature or higher (24-50 degrees C).