Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis
Stig Pedersen-Bjergaard, Ellen Sannes, Knut Rasmussen, Finn Tønnesen
Journal of Chromatography. B, Biomedical Sciences and Applications July 1, 1997 DOI: 10.1016/s0378-4347(97)00127-8 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Population | Psilocybe semilanceata mushrooms |
| Topics | Psilocybin |
| Keywords | Capillary electrophoresis Chromatography |
| Citations | 28 |
| Key points | A capillary zone electrophoresis method was developed that rapidly and precisely quantifies psilocybin and baeocystin in Psilocybe semilanceata. |
Abstract
A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by UV spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.
Citations in the library
Cited by 16
- Fungal Hallucinogens Psilocin, Ibotenic Acid, and Muscimol Therapeutic Drug Monitoring July 12, 2013
- The Determination of Psilocin and Psilocybin in Hallucinogenic Mushrooms by HPLC Utilizing a Dual Reagent Acidic Potassium Permanganate and Tris(2,2′‐bipyridyl)ruthenium(II) Chemiluminescence Detection System Journal of Forensic Sciences 2006
- Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids TemaNord May 19, 2009
- A journey with psychedelic mushrooms: From historical relevance to biology, cultivation, medicinal uses, biotechnology, and beyond Biotechnology Advances August 31, 2023
- Psilocybin for Treating Psychiatric Disorders: A Psychonaut Legend or a Promising Therapeutic Perspective? Journal of Xenobiotics February 7, 2022
and 11 more in the library
Cites 8
- Occurrence of Psilocybin in Various Higher Fungi from Several European Countries Planta Medica October 1, 1985
- Ethnomycology, biochemistry, and cultivation of Psilocybe samuiensis Guzmán, Bandala and Allen, a new psychoactive fungus from Koh Samui, Thailand. Journal of Ethnopharmacology July 8, 1994
- Psychoactive mushroom use in Koh Samui and Koh Pha-Ngan, Thailand. Journal of Ethnopharmacology 1992
- Occurrence of Psilocybin/Psilocin in Pluteus Salicinus (Pluteaceae) Mycologia July 1, 1981
- The Content of Psilocybin in Norwegian Psilocybe semilanceata Planta Medica July 1, 1981
and 3 more in the library