Towards a molecular approach for the identification of fungal taxa that contain psilocybin
Lee Jc
Study at a glance
AI-extracted from the abstract| Characteristics | Observational study Peer reviewed |
|---|---|
| Sample size | 20 |
| Population | Twenty samples of Psilocybe and Panaeolus mushrooms known to produce psilocybin |
| Topics | Psilocybin |
| Keywords | Identification biology Taxon Evolutionary biology Computational biology Ecology Hallucinogen |
| Citations | 2 |
| Key findings | RAPD and AFLP molecular profiling can reliably identify genus and species of psilocybin-producing mushrooms, overcoming limitations of morphological taxonomy. |
Abstract
The classical taxonomic methodologies for determining taxa of mushrooms are based primarily on morphological traits. If the mushroom specimens are dried or pulverized, the morphological characteristics are frequently disguised or no longer apparent. Included among these fungi are known to produce psilocybin, which are hard to identify by their macroscopic features. Molecular techniques could help to overcome these problems. In this study, twenty samples of two genera, Psilocybe and Panaeolus, of fungi from genera known to produce psilocybin were analyzed. The profiles of random amplified polymorphic DNA (RAPD) produced by two primer sets were analyzed. Fingerprints of amplified fragment length polymorphism (AFLP) produced by five selective primer pairs were also analyzed. By the analysis of genetic similarity, RAPD and AFLP method were capable of providing genus and species information. AFLP band patterns can provide reliable species test and can be separated in a simple way using PAGE electrophoresis and stained by silver nitrate. The sequencing data of twenty psilocybin-producing mushrooms were established by fluorescent sequencing method on the nuclear small subunit ribosomal DNA (nuc-ssu-rDNA) and the ... (continues)
Comparable studies
Other observational and cohort studies on psilocybin, most cited first.
| Study | Year | Design | Participants |
|---|---|---|---|
| Psilocybin for treatment-resistant depression: fMRI-measured brain mechanisms. Patients with treatment-resistant depression | 2017 | Observational cohort | n = 19 |
| Psychedelic effects of psilocybin correlate with serotonin 2A receptor occupancy and plasma psilocin levels Healthy volunteers | 2019 | Observational cohort | n = 8 |
| Increased spontaneous MEG signal diversity for psychoactive doses of ketamine, LSD and psilocybin Humans | 2017 | Observational study | |
| Classic psychedelic use is associated with reduced psychological distress and suicidality in the United States adult population. USA adult respondents from the National Survey on Drug Use and Health (2008-2012) | 2015 | Observational cohort | n = 190,000 |
| Prediction of Psilocybin Response in Healthy Volunteers Healthy volunteers | 2012 | Observational cohort | n = 409 |