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Psilocybin reduces functional connectivity and the encoding of spatial information by neurons in mouse retrosplenial cortex

Victorita E. Ivan, David P Tomàs-Cuesta, Ingrid M. Esteves, Artur Luczak, Majid Mohajerani, Bruce L McNaughton, Aaron J. Gruber

April 22, 2024 DOI: 10.22541/au.171378690.00112411/v1 (opens in new tab)

Study at a glance

AI-extracted from the abstract
Characteristics Experimental study
Population Head-fixed mice navigating on a treadmill
Interventions Psilocybin Ketanserin
Topics Psilocybin
Keywords Retrosplenial cortex Hallucinogen Perception Cortex anatomy
Citations 1
Key findings Psilocybin reduced the place specificity, stability, and functional connectivity of neural activity in the retrosplenial cortex of mice, effects largely blocked by ketanserin.

Abstract

Psychedelic drugs have profound effects on perception, cognition, and mood. How psychedelics affect neural signaling to produce these effects remains poorly understood. We investigated the effect of the classic psychedelic psilocybin on neural activity patterns and spatial encoding in the retrosplenial cortex of head-fixed mice navigating on a treadmill. The place specificity of neurons to distinct locations along the belt was reduced by psilocybin. Moreover, the stability of place-related activity across trials decreased. Psilocybin also reduced the functional connectivity among simultaneously recorded neurons. The 5-HT2AR (serotonin 2A receptor) antagonist ketanserin blocked the majority of these effects. These data are consistent with proposals that psychedelics increase the entropy of neural signaling, and provide a potential neural mechanism contributing to disorientation frequently reported by humans after taking psychedelics.

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