Preparation of Monoclonal Antibodies Reactive to a Hallucinogenic Drug, Psilocin
Mitsune Yamaguchi, Toshiaki Saito, Yoshie Horiguchi, Keita Ogawa, Yukihiro Tsuchiya, Kayoko Hishinuma, Toshiyuki Chikuma, Yukiko Makino, Hiroshi Hojo
JOURNAL OF HEALTH SCIENCE 2004 DOI: 10.1248/jhs.50.600 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory experiment Peer reviewed |
|---|---|
| Population | BALB/c mice |
| Topics | Psilocybin |
| Keywords | Monoclonal antibody Hallucinogen Keyhole limpet hemocyanin Immunoassay Molecular biology Biochemistry |
| Citations | 5 |
| Key findings | Monoclonal antibody BA631 reacts with psilocin and dimethyltryptamine but not with psilocybin or other indole derivatives, enabling potential identification of magic mushrooms. |
Abstract
The cultivation or trafficking of “Magic mushrooms,” containing hallucinogenic psilocin and psilocybin, has been prohibited by the Narcotics and Psychotropics Control Law in Japan since 2002. To identify these mushrooms, we attempted to prepare the monoclonal antibody (mAb) reactive to these hallucinogens. As an antigen inducing an anti-psilocin mAb, N-{4-[3-(2-dimethylaminoethyl)indol-4-yl-oxy]butyl}succinamic acid was synthesized by modifying the 4-hydroxyl moiety of psilocin and coupled to a carrier protein of keyhole limpet hemocyanin. BALB/c mice were immunized five times with the antigen emulsified with an adjuvant, and their spleen cells were fused with mouse myeloma cells. We obtained several hybridoma cells producing mAbs reactive to psilocin, from which four clones, BA631, CA231, KA422, and MA332 with a higher production of anti-psilocin mAb were selected by limiting dilution. Isotype of CA231 and KA422 mAbs were IgG2a and that of BA631 and MA332 mAbs were IgG1. Enzyme immunoassay (EIA) using BA631 mAb, revealed that BA631 cross-reacted with psilocin and dimethyltryptamine, but not with the other indole derivatives such as psilocybin, 4-hydroxyindole, tryptamine, and tryptophan. Therefore, these antibodies could be used for the identification of magic mushrooms.
Comparable studies
Other preclinical and animal studies on psilocybin, most cited first.
| Study | Year | Design | Participants |
|---|---|---|---|
| Psilocybin induces rapid and persistent growth of dendritic spines in frontal cortex in vivo Mice | 2021 | Observational cohort | |
| Harnessing psilocybin: antidepressant-like behavioral and synaptic actions of psilocybin are independent of 5-HT2R activation in mice Mice | 2021 | Experimental study | |
| Psychedelics reopen the social reward learning critical period Mice | 2023 | Experimental study in mice | |
| Correlation between the potency of hallucinogens in the mouse head-twitch response assay and their behavioral and subjective effects in other species. C57BL/6J mice | 2020 | Experimental study with dose-response experiments | |
| Effects of psilocybin on hippocampal neurogenesis and extinction of trace fear conditioning Mice | 2013 | Experimental study |
Citations in the library
Cited by 2
- Effect of Psilocin on Extracellular Dopamine and Serotonin Levels in the Mesoaccumbens and Mesocortical Pathway in Awake Rats Biological and Pharmaceutical Bulletin October 23, 2014
- Immunochemical monitoring of psilocybin and psilocin to identify hallucinogenic mushrooms Journal of Pharmaceutical and Biomedical Analysis July 21, 2020
Cites 5
- Concise Large-Scale Synthesis of Psilocin and Psilocybin, Principal Hallucinogenic Constituents of “Magic Mushroom” Journal of Natural Products May 30, 2003
- Hallucinogenic mushrooms on the German market - simple instructions for examination and identification. Forensic Science International September 11, 2000
- Quantitative analysis of psilocybin and psilocin and psilocybe baecystis (singer and smith) by high-performance liquid chromatography and by thin-layer chromatography Journal of Chromatography A March 1, 1981
- Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis Journal of Chromatography. B, Biomedical Sciences and Applications July 1, 1997
- Synthesis of a psilocin hapten and a protein-hapten conjugate Journal of Pharmacy and Pharmacology September 1, 2002