Determination of common drugs of abuse in body fluids using one isolation procedure and liquid chromatography--atmospheric-pressure chemical-ionization mass spectromery.
M J Bogusz, R D Maier, K D Krüger, U Kohls
Journal of Analytical Toxicology 1998 DOI: 10.1093/jat/22.7.549 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Topics | LSD |
| Keywords | Drug screening Drug testing Chemical analysis Contaminant detection Illicit substances Opiate agonists Cocaine metabolites Forensic science: criminalistics Legal science Forensic toxicology Crime lab analysis Forensic analysis Mass spectrometry: ms Lc-ms Gc-ms Chemical instrumentation Analytical techniques Advanced mass spectrometry Body fluid analysis Clinical samples Biological matrices Biospecimen analysis Biological samples Blood Urine Other body fluids Sample preparation Purification techniques Separation science Single Universal extraction method |
| Citations | 111 |
| Key points | A common solid-phase extraction method using C18 cartridges provides high recoveries and clean extracts for a broad range of drugs from various biological matrices, suitable for routine forensic analysis. |
Abstract
A method for determining opiate agonists (morphine, morphine-3-glucuronide, morphine-6-glucuronide, 6-monoacetylmorphine, codeine, codeine-6-glucuronide, dihydrocodeine, dihydromorphine, buprenorphine, methadone, tramadol, and ibogaine), cocaine and its metabolites (benzoylecgonine and ecgonine methyl ester) and lysergic acid diethylamide in serum, blood, urine and other biological matrices is presented. Aliquots (0.5-1.5 mL) of biological fluids were spiked with appropriate deuterated internal standards and extracted using a common solid-phase extraction method (C18 cartridges). The extracts were subjected to liquid chromatographic-atmospheric-pressure chemical-ionization mass spectrometric examination using selected ion monitoring procedures. These procedures were developed after analysis of full-scan mass spectra of examined compounds. The extraction method appeared very universal; the recoveries were high for almost all drugs and the extracts were very clean. The procedure was applied for routine forensic casework.