Simultaneous determination of thirteen plant alkaloids in a human specimen by SPE and HPLC.
Jörg Pietsch, Jana Günther, Thomas Henle, Jan Dressler
Journal of Separation Science July 1, 2008 DOI: 10.1002/jssc.200800128 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Population | Human specimens (forensic and clinical samples) |
| Keywords | Analytical methods Toxicology Forensic science Clinical diagnostics |
| Citations | 56 |
| Key points | A validated solid-phase extraction and liquid chromatography method enables simultaneous detection and quantification of thirteen plant alkaloids in human specimens for forensic and clinical applications. |
Abstract
A new screening method for the simultaneous determination of thirteen plant alkaloids (aconitine, anabasine, atropine, brucine, colchicine, cotinine, cytisine, harmine, ibogaine, nicotine, scopolamine, strychnine, yohimbine) in a human specimen was developed based on solid-phase extraction and reversed-phase liquid chromatography with photodiode array detection. The validated method enables selective identification as well as accurate and sensitive quantification. The analysis of forensic and clinical samples emphasizes the applicability for intoxications and drug abuse, as well as for compliance control.