A new screening method using solid-phase extraction and liquid chromatography with photodiode array detection can simultaneously identify and quantify thirteen plant alkaloids in human samples. The validated technique provides selective, accurate, and sensitive measurements of these compounds, which include both toxic and medicinal substances. Analysis of forensic and clinical specimens demonstrates the method's usefulness for detecting intoxications, monitoring drug abuse, and checking patient compliance with prescribed medications.
Optimal separation and online concentration methods for nine tryptamines, including DMT, AMT, and 5-MeO-DMT, were developed using micellar electrokinetic chromatography with UV detection. Limits of detection ranged from 1.0 to 1.8 μg/mL with standard MEKC, improved to 2.2–8.0 ng/mL using sweeping-MEKC, and further to 1.3–2.7 ng/mL using cation-selective exhaustive injection-sweep-MEKC. The migration order of the tryptamines was determined. A spiked human urine sample containing DMT was successfully analyzed, demonstrating practical applicability for drug testing.