A multi-component LC-MS/MS method for detection of ten plant-derived psychoactive substances in urine.
Kristian Björnstad, Olof Beck, Anders Helander
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences April 15, 2009 DOI: 10.1016/j.jchromb.2009.03.004 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Keywords | Psychoactive compound screening Toxin identification Drug testing Intoxicant detection Compound detection Substance detection Psychoactive substances Compounds Urinalysis urine testing Biological fluid analysis Urine screening Diagnostic urinalysis Urine sample analysis Samples Intoxication diagnosis Emergency toxicology Poisoning diagnosis Clinical intoxication Rapid diagnosis Assay method Diagnostic procedure Screening method Analysis technique Robust technique Breakthrough method |
| Citations | 71 |
| Key points | The developed LC-MS/MS method can simultaneously detect ten plant-derived psychoactive substances in urine with good linearity and precision, and is suitable for clinical intoxication cases. |
Abstract
A sensitive and specific LC-MS/MS method for simultaneous detection of 10 plant-derived psychoactive substances (atropine, N,N-dimethyltryptamine, ephedrine, harmaline, harmine, ibogaine, lysergic acid amide, psilocin, scopolamine and yohimbine) in urine was developed. Direct injection of urine diluted with 3 deuterated internal standards allowed for a readily accessible method suitable for application in clinical intoxication cases. Separation was achieved using reversed phase chromatography and gradient elution with a total analysis time of 14 min. Electrospray ionization was used and ions were monitored in the positive selected reaction monitoring mode. The calibration curves were linear (r(2)>0.999) and the total imprecision at high (1000 microg/L) and low (50 microg/L) substance concentrations were 4.9-13.8% and 8.3-26%, respectively. Infusing the analytes post column and injecting matrix samples showed limited influence by ion suppression. The multi-component method proved to be useful for investigation of authentic cases of intoxication with plant-derived psychoactive drugs and was indicated to cover the clinically relevant concentration ranges.