Comparison of fragrance and flavor components in non-psilocybin and psilocybin mushrooms using vacuum-assisted headspace high-capacity solid-phase microextraction and gas chromatography–mass spectrometry
Advances in Sample Preparation September 2, 2023 Shannon L. Thomas, Colton Myers, Kevin A Schug 13 citations
Vacuum-assisted headspace high capacity solid-phase microextraction (Vac-HS-HC-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) was used to compare volatile compounds in five psilocybin mushrooms (Psilocybe cubensis) and three non-psilocybin mushroom species. Common volatiles included acids, alcohols, aldehydes, ketones, and hydrocarbons. Vac-HS-HC-SPME detected twice the compound response and eight additional compounds compared to standard HS-HC-SPME. Compounds unique to psilocybin mushrooms were 2-methylbutanal, valeraldehyde, benzaldehyde, 3-octen-2-one, 2-methyl-dodecane, and 2‑butyl‑2-octenal. Compounds unique to non-psilocybin mushrooms were 2-methyl-pyrazine, 2,3-butanediol, butyric acid, butyrolactone, benzyl alcohol, 2-pyrrolidinone, and estragole. The shared compound 1-octen-3-ol showed higher response in psilocybin mushrooms.