This study evaluates the feasibility of near-infrared (NIR) spectroscopy for identifying biochemical differences in Nicotiana species (N. spp.) in ceremonial, handcrafted Indigenous smokeless tobacco products from Ecuador and Peru. NIR spectra of three N. spp. Nicotiana tabacum (N. tabacum), Nicotiana glauca (N. glauca), Nicotiana rustica, (N. rustica) and three smokeless tobacco samples (SLT),...