Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography
Electrophoresis March 1, 2003 Ching Fang, Ju-Tsung Liu, S.‐s. Chou et al. 27 citations
Capillary electrophoresis combined with fluorescence spectroscopy, using sodium dodecyl sulfate as a surfactant, separates and concentrates lysergic acid diethylamide (LSD) in mouse blood. Two on-line concentration techniques—sweeping micellar electrokinetic chromatography and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography—were optimized. In a test mouse fed 0.1 mg LSD (about one-tenth the LD50 value for a 20 g mouse), LSD concentrations of 120 ng/mL and 30 ng/mL were detected in blood at 20 and 60 minutes after ingestion, respectively.