Surgical Protocol for a Large, Resealable Cranial Window Enabling Longitudinal, Multi-Modal Electrophysiology Recordings of Mouse Default Mode Network.
Journal of visualized experiments : JoVE May 29, 2026 Tommi Kiiso, Paula Partanen, Keyrstin Jacobs et al.
A new surgical protocol creates a large, durable, resealable cranial window in mice that preserves the dura mater, enabling stable, long-term recordings from over 1,000 electrodes. This technique allows simultaneous surface and deep brain recordings from the default mode network over several weeks, offering a platform to study network-wide brain dynamics and evaluate chronic effects of treatments for disorders like major depressive disorder.