IL-1 beta and TNF alpha modulate delta 9-tetrahydrocannabinol-induced catalepsy in mice.
M Gibertini, C Newton, H Friedman, T W Klein
Pharmacology, Biochemistry, and Behavior February 1995 DOI: 10.1016/0091-3057(94)00270-s (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental animal study Peer reviewed |
|---|---|
| Population | Mice |
| Interventions | THC recombinant IL-1 beta anti-TNF alpha antibodies recombinant IL-1 alpha |
| Dose | THC 10 micrograms/mouse (low dose) or 75 micrograms/mouse (high dose), IV; recombinant IL-1 beta 400 ng/mouse, IV; recombinant TNF alpha 500 ng/mouse, IV |
| Topics | Cannabis |
| Key findings | Recombinant IL-1 beta and TNF alpha potentiated low-dose THC-induced catalepsy, while antibodies to IL-1 beta and TNF alpha attenuated high-dose THC-induced catalepsy; IL-1 alpha and IL-6 had no effect. The authors suggest that IL-1 beta and TNF alpha regulate early and late THC-induced catalepsy by different mechanisms. |
Abstract
The role of the proinflammatory cytokines interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF alpha) in THC-induced catalepsy in mice was examined. Recombinant IL-1 beta (400 ng/mouse, IV) and TNF alpha (500 ng/mouse, IV) were effective in potentiating the cataleptic effect of low-dose THC (10 micrograms/mouse, IV). Recombinant IL-1 alpha and IL-6 did not potentiate catalepsy at any dose tested. Anti-IL-1 beta and anti-TNF alpha antibodies were effective in attenuating high-dose (75 micrograms/mouse) THC-induced catalepsy. Antibodies to IL-1 alpha and IL-6 had no effect on catalepsy. Early onset catalepsy (10 min postinjection) was potentiated by exogenous recombinant IL-1 beta and TNF alpha but only later catalepsy (2 h postinjection) was attenuated by antibodies to endogenous IL-1 beta or TNF alpha. This divergence of the cytokine effect suggests that these substances regulate, by different mechanisms, the early and late THC-induced cataleptic response.
Comparable studies
Other preclinical and animal studies on cannabis, most cited first.