Phytochemical investigation of natural and in vitro raised Vṛddhadāruka plants.
Asha Jyoti Bharati, Yogendra Kumar Bansal
Ancient Science of Life 2014 DOI: 10.4103/0257-7941.153463 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro experimental plant tissue culture and phytochemical screening study Peer reviewed |
|---|---|
| Population | Argyreia nervosa (elephant creeper) nodal explants and extracts of seeds, natural and in vitro leaves, stem, and callus |
| Intervention | 6-benzylaminopurine |
| Keywords | 6-benzylaminopurine Argyreia nervosa Endangered In vitro regeneration Phytochemical |
| Key findings | Different concentrations of 6-benzylaminopurine induced shoot and root initiation in cultured nodal explants. Phytochemical screening of extracts from seeds, natural and in vitro leaves, stem, and callus detected bioactive substances such as flavonoids, alkaloids, and terpenoids. The authors propose the protocol as an alternative method to conserve the plant and suggest its secondary metabolites may be useful for treating various diseases. |
Abstract
Argyreia nervosa commonly known as elephant creeper (English) and Vṛddhadāruka (Sanskrit) is a woody climber that belongs to the family Convolvulaceae. Seeds of this plant contain hallucinogens including ergot alkaloids and a naturally occurring lysergic acid amide. Traditionally the plant is used in the treatment of gonorrhea, strangury, chronic ulcers, diabetes, anemia and cerebral disorders. The plant is also used as appetitiser, brain tonic, cardiotonic, aphrodisiac. It possesses anti-inflammatory, immunomodulatory, antibacterial, antiviral and antifungal activities. To give an account of information on in vitro regeneration and phytochemical analysis of the plant. Nodal explants were selected for in vitro regeneration. Different aerial parts viz., seeds, natural and in vitro leaf, stem and callus were dried and extracted with different solvents and were subjected to various phytochemical analyses. Different concentrations of 6-benzylaminopurine showed shoot and root initiation. The study of phytochemical screening of different extracts showed the presence of bioactive substances like flavonoids, alkaloids, terpenoids, etc. The study will provide an efficient in vitro protocol for micropropagation as an alternative method to conserve the plant and shows the presence of some important secondary metabolites in the nature grown and in vitro raised plants which can be useful for treatment of various diseases.