PS125. Functional and morphological changes induced by ketamine in the hippocampus
G. Treccani, Liebenberg N. Nico, S. Tillmann, Laura Musazzi, Marco Milanese, Feng-Hua Chen, Betina Elfving, Gregers Wegener, Jens R Nyengaard, Maurizio Popoli, H. Müller
The International Journal of Neuropsychopharmacology May 27, 2016 DOI: 10.1093/ijnp/pyw043.125 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Preclinical animal study Peer reviewed |
|---|---|
| Population | Mice |
| Interventions | Desipramine Venlafaxine Bupropion Imipramine Diazepam |
| Dose | Imipramine 30 mg/kg; desipramine 7.5 mg/kg and 15 mg/kg; venlafaxine 4 mg/kg and 8 mg/kg; bupropion 20 mg/kg and 40 mg/kg; diazepam 2 mg/kg |
| Measures | Forced swimming test (FST), elevated plus maze test (EPM), open field test |
| Topics | Esketamine Ketamine |
| Key points | Desipramine, venlafaxine, and bupropion produced dose-dependent antidepressant-like and anxiolytic-like effects in mice, generally comparable to imipramine and diazepam; bupropion at 40 mg/kg was more effective than both reference drugs, while none of the drugs changed locomotor activity in the open field test. |
Abstract
Specific objective of the study: This study is aimed to investigate desipramine, venlafaxine and bupropion on depression and anxiety in mice. Methods used: We used a classic tricyclic antidepressant, desipramine; a serotonin-norepinephrine reuptake inhibitör, venlafaxine; a second-generation antidepressant agent that blocks centrally the reuptake of noradrenaline and dopamine, bupropion in forced swimming test (FST), elevated plus maze test (EPM) and open field test. Summary of results: Imipramine (30 mg/kg), desipramine (7.5 mg/kg and 15 mg/kg), venlafaxine (4 mg/kg and 8 mg/kg), bupropion (20 mg/kg and 40 mg/kg) significantly reduced immobility time dose dependently, compared to vehicle-treated group. And also bupropion (40 mg/kg) exerted significant antidepressant-like effects in the mice FST which was more effective than imipramine. Diazepam (2 mg/kg), desipramine (7.5 mg/kg and 15 mg/kg), venlafaxine (4 mg/kg and 8 mg/kg), bupropion (20 mg/kg and 40 mg/ kg) significantly increased the time spent in the open arms dose dependently, compared to the vehicle-treated group. Diazepam (2 mg/kg), desipramine (7.5 mg/kg and 15 mg/kg), venlafaxine 8 mg/kg) and bupropion (20 mg/kg and 40 mg/kg) significantly increased the number of entries into the open arms, compared to the vehicle-treated group. And also bupropion (40 mg/kg) exerted significant anxiolytic-like effects in the mice EPM test which was more effective than diazepam. None of the drugs modified the total distance moved in the open field test. Conclusions reached: desipramine, venlafaxine and bupropion (20 mg/kg) exerted significant antidepressant-like effects which was as effective as imipramine. And also bupropion (40 mg/kg) exerted significant antidepressant-like effects which was more effective than imipramine. Again, desipramine, venlafaxine and bupropion (20 mg/kg) exerted significant anxiolytic-like effects which was as effective as diazepam. And also bupropion (40 mg/ kg) exerted significant anxiolytic-like effects which was more effective than diazepam. PS125 Functional and morphological changes induced by ketamine in the hippocampus Giulia Treccani1,2, Nico Liebenberg N1, Sandra Tillmann1, Laura Musazzi2, Marco Milanese3, Fenghua Chen4, Betina Elfving1, Gregers Wegener1,5, Jens Randel Nyengaard4, Maurizio Popoli2, Heidi Kaastrup Müller1. 1 Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov, Denmark 2 Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento di Scienze Farmacologiche e Biomolecolari and Center of Excellence on Neurodegenerative Diseases (CEND), Università degli Studi di Milano, Milan, Italy 3 Department of Pharmacy, Unit of Pharmacology and Toxicology, University of Genova, Genoa, Italy. 4 Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, Aarhus, Denmark 5 Pharmaceutical Research Center of Excellence, School of Pharmacy, North-West University, Potchefstroom, South
Comparable studies
Other preclinical and animal studies on ketamine, most cited first.
| Study | Year | Design | Participants |
|---|---|---|---|
| mTOR-Dependent Synapse Formation Underlies the Rapid Antidepressant Effects of NMDA Antagonists Rats | 2010 | Observational study | |
| Activation of Glutamatergic Neurotransmission by Ketamine: A Novel Step in the Pathway from NMDA Receptor Blockade to Dopaminergic and Cognitive Disruptions Associated with the Prefrontal Cortex Conscious rats | 1997 | Dose-response study with microdialysis and behavioral testing | |
| NMDAR inhibition-independent antidepressant actions of ketamine metabolites Mice | 2016 | Preclinical study | |
| The dissociative anaesthetics, ketamine and phencyclidine, selectively reduce excitation of central mammalian neurones by N‐methyl‐aspartate Spinal neurons in decerebrate or pentobarbitone-anaesthetized cats and rats | 1983 | Experimental study | |
| R-ketamine: a rapid-onset and sustained antidepressant without psychotomimetic side effects Mice | 2015 | Experimental study |