Effects of a combination of 3,4-methylenedioxymeth amphetamine and caffeine on real time stimulated dopamine release in the rat striatum: Studies using fast cyclic voltammetry.
J J O'Connor, K M O'Boyle, J P Lowry
Journal of Neuroscience Methods April 15, 2018 DOI: 10.1016/j.jneumeth.2017.08.026 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro experimental study Peer reviewed |
|---|---|
| Population | Isolated striatal slices from adult male Wistar rats |
| Interventions | Caffeine MDMA |
| Dose | 10 or 100 µM caffeine; 1 or 30 µM MDMA |
| Measures | fast cyclic voltammetry |
| Topics | MDMA |
| Keywords | Caffeine Cyclic voltammetry Rat striatum Single pulse |
| Key findings | Caffeine at 100 µM increased 5-pulse but not single-pulse stimulated dopamine release, while MDMA at 1 and 30 µM increased both single- and 5-pulse release and reuptake. Combining 100 µM caffeine with MDMA did not significantly enhance MDMA's effects on dopamine release or reuptake, so the results do not demonstrate a caffeine-enhanced MDMA-induced dopamine release. |
Abstract
It is well documented that caffeine exacerbates the hyperthermia associated with acute exposure to 3,4-methylenedioxymethamphetamine (MDMA) in rats. Previous reports have also indicated that MDMA-related enhancement of dopamine release is exacerbated in the presence of caffeine. In the present study we have examined whether the effects of MDMA on real-time stimulated dopamine release, in the absence of uptake inhibition, are accentuated in the presence of caffeine. Isolated striatal slices from adult male Wistar rats were treated acutely with MDMA, caffeine, or a combination, and their effects on single and 5pulse stimulated dopamine release monitored using the technique of fast cyclic voltammetry. Caffeine at 10 or 100μM had no significant effect on single pulse stimulated dopamine release. However 100μM caffeine caused a significant peak increase in 5pulse stimulated dopamine release. Both 1 and 30μM MDMA gave rise to a significant increase in both single and 5-pulse dopamine release and reuptake. A combination of 100μM caffeine and 1 or 30μM MDMA did not significantly enhance the effects of MDMA on single or 5pulse dopamine release and reuptake when compared to that applied alone. Utilizing single action potential dependent dopamine release, these results do not demonstrate a caffeine-enhanced MDMA-induced dopamine release.
Comparable studies
Other preclinical and animal studies on MDMA, most cited first.
Citations in the library
Cites 15
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and 10 more in the library