FABP5 is important for cognitive function and is an important regulator of the physiological effects and pharmacokinetics of acute Δ9 tetrahydrocannabinol inhalation in mice.
Samantha L Penman, Nicole M Roeder, Erin C. Berthold, Alexandria S. Senetra, Matthew Marion, Brittany J Richardson, Olivia White, Nathan L Fearby, Christopher R Mccurdy, John Hamilton, Abhisheak Sharma, Panayotis K Thanos
Pharmacology, Biochemistry, and Behavior October 2023 DOI: 10.1016/j.pbb.2023.173633 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Observational animal study Peer reviewed |
|---|---|
| Population | Adult FABP5+/+ and FABP5-/- mice |
| Intervention | Δ9-tetrahydrocannabinol (THC) |
| Measures | Open Field (OF), Novel Object Recognition (NOR), T-Maze, Morris Water Maze (MWM), Elevated Plus Maze (EPM), UPLC-MS/MS |
| Topics | Cannabis |
| Keywords | Endocannabinoid system Locomotion Memory Pharmacokinetics Δ9-tetrahydrocannibinol |
| Key findings | The authors report that some cognitive deficits from FABP5 deletion, particularly in the Morris Water Maze, were blocked by THC administration, while other memory and anxiety measures (T-Maze and Elevated Plus Maze) were not. They also found that FABP5 alters the pharmacokinetics of THC, indicating it regulates THC's physiological effects. |
Abstract
Fatty acid binding protein 5 (FABP5) interacts with the endocannabinoid system in the brain via intracellular transport of anandamide, as well as Δ9-tetrahydrocannabinol (THC), the main psychoactive component of cannabis. Previous work has established the behavioral effects of genetic deletion of FABP5, but not in the presence of THC. The present study sought to further elucidate the role of FABP5 on the pharmacokinetic and behavioral response to THC through global deletion. Adult FABP5+/+ and FABP5-/- mice were tested for behavioral response to THC using Open Field (OF), Novel Object Recognition (NOR), T-Maze, Morris Water Maze (MWM), and Elevated Plus Maze (EPM). An additional cohort of mice was used to harvest blood, brains, and liver samples to measure THC and metabolites after acute administration of THC. Behavioral tests showed that some cognitive deficits from FABP5 deletion, particularly in MWM, were blocked by THC administration, while this was not observed in other measures of memory and anxiety (such as T-Maze and EPM). Measurement of THC and metabolites in blood serum and brain tissue through UPLC-MS/MS analysis showed that the pharmacokinetics of THC was altered by FABP5. The present study shows further evidence of the importance of FABP5 in cognitive function. Additionally, results showed that FABP5 is an important regulator of the physiological effects and pharmacokinetics of THC.
Comparable studies
Other preclinical and animal studies on cannabis, most cited first.
Citations in the library
Cites 11
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- Pharmacokinetics and central accumulation of delta-9-tetrahydrocannabinol (THC) and its bioactive metabolites are influenced by route of administration and sex in rats. Scientific Reports December 14, 2021
- Biphasic effects of THC in memory and cognition. European journal of clinical investigation May 2018
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