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The PrLGlu→avBNSTGABA circuit rapidly modulates depression-like behaviors in male mice.

Jie-Ying Chen, Ke Wu, Miao-Miao Guo, Wei Song, Si-Ting Huang, Yong-Mei Zhang

iScience October 20, 2023 DOI: 10.1016/j.isci.2023.107878 (opens in new tab)

Study at a glance

AI-extracted from the abstract
Characteristics Preclinical animal study Peer reviewed
Population Mice exposed to chronic restraint stress with individual housing
Interventions Ketamine chemogenetic activation
Topics Depression
Keywords Anesthesiology
Key points Activating the PrLGlu→avBNSTGABA circuit had antidepressant effects in depression-like mice, with AMPA receptors playing a dominant role. Ketamine rapidly alleviated depression-like behaviors and rescued decreased activity in this circuit, and ketamine's fast behavioral effect was diminished when the circuit was inhibited. The authors propose the circuit as a target for fast regulation of depression.

Abstract

Depression is a global disease with a high prevalence. Here, we examine the role of the circuit from prelimbic mPFC (PrL) to the anterior ventral bed nucleus of the stria terminalis (avBNST) in depression-like mice through behavioral tests, immunofluorescence, chemogenetics, optogenetics, pharmacology, and fiber photometry. Mice exposed to chronic restraint stress with individual housing displayed depression-like behaviors. Optogenetic or chemogenetic activation of the avBNST-projecting glutamatergic neurons in the PrL had an antidepressant effect. Moreover, we found that α-amino-3-hydroxy-5-methyl-4-isoxazole-propionicacid receptors (AMPARs) play a dominant role in this circuit. Systemic administration of ketamine profoundly alleviated depression-like behaviors in the mice and rapidly rescued the decreased activity in the PrLGlu→avBNSTGABA circuit. Furthermore, the fast-acting effect of ketamine on depressive behaviors was diminished when the circuit was inhibited. To summarize, activating the PrLGlu→avBNSTGABA circuit quickly ameliorated depression-like behaviors. Thus, we propose the PrLGlu→avBNSTGABA circuit as a target for fast regulation of depression.

Comparable studies

Other preclinical and animal studies on depression, most cited first.

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