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Detection of Psychoactive N,N-Dimethyltryptamine Alkaloid Based on Surface‑Enhanced Raman Scattering Using Gold Nanostars in Flexible Inkjet‑Printing Paper Substrates

Edison Huertas Montoya, Anerise De Barros, Lucas C Oliveira, Paola Caro Aponte, Alexandre L R Oliveira, Flavio Shimizu, Alessandra Sussulini, Fernando Sigoli, Italo O. Mazali

Journal of the Brazilian Chemical Society 2024 DOI: 10.21577/0103-5053.20240105 (opens in new tab)

Study at a glance

AI-extracted from the abstract
Characteristics Proof-of-concept laboratory analytical study Peer reviewed
Measures surface-enhanced Raman scattering (SERS), principal component analysis (PCA), silhouette coefficient
Topics 5-MeO-DMT DMT
Key points The gold nanostar inkjet-printed paper substrate detected DMT in water across concentrations from 10-3 to 10-10 mol L-1, with one print cycle sufficient for high performance. Principal component analysis of the SERS spectra produced a silhouette coefficient of 0.84, which the authors present as evidence of the sensor's sensitivity and potential for monitoring DMT levels in therapeutic or biological contexts.

Abstract

N,N-Dimethyltryptamine (DMT) is a serotonergic psychedelic that, when combined with monoamine oxidase enzyme inhibitors in the hallucinogenic beverage commonly known as ayahuasca, surpasses most common orally administered psychoactive drugs. There is a growing interest in the therapeutic potential of DMT due to recent clinical data showing the improvement of cognitive deficits associated with depression and Alzheimer’s disease. The development of analytical methodology for the monitoring of concentrations of DMT molecules to control the appropriate dosage for their use is essential for the treatment of patients. For this, we propose a novel flexible inkjet-printed paper substrate based on Au nanostars as proof of concept for DMT detection by surface-enhanced Raman scattering (SERS). The substrate was applied to detect DMT in water as a first approach to more complex matrices like ayahuasca beverages or blood samples. The optimization and performance of SERS substrates with different print cycles indicate that one print is enough to achieve the great performance of the flexible paper substrates. The SERS analyses for DMT in different concentrations, 10-3 to 10-10 mol L-1, reveal a high sensitivity of the sensors with a silhouette coefficient of 0.84 obtained by principal component analysis (PCA) statistical projections.