Dose-dependent effects of phencyclidine on hippocampal CA1 neurons.
Canadian journal of physiology and pharmacology May 1987 DOI: 10.1139/y87-135 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro electrophysiological study Peer reviewed |
|---|---|
| Population | Guinea pig hippocampal slices, CA1 pyramidal neurons |
| Intervention | Phencyclidine |
| Dose | 0.2-0.4 microM (low), 2.0-10.0 microM (medium), 0.01-10 mM (high) |
| Duration | Effects developed over 15-30 min; washout up to 1 h |
| Measures | orthodromically evoked population potentials, intracellular recordings, spontaneous firing rates |
| Key points | Phencyclidine acts on guinea pig CA1 hippocampal neurons in a dose-dependent manner: low doses (0.2-0.4 microM) enhance evoked population potentials and increase firing, high doses (0.01-10 mM) depress them, and medium doses (2.0-10.0 microM) have little effect on population potentials while producing mixed firing changes. Effects develop over 15-30 minutes and are only partially reversible after up to one hour of washout. |
Abstract
The dose-dependent effects of phencyclidine were examined in guinea pig hippocampal slices using intracellular and extracellular recordings. Orthodromically evoked population potentials from the CA1 cell body layer were enhanced by low doses (0.2-0.4 microM) and depressed by high doses (0.01-10 mM). Medium doses of the drug (2.0-10.0 microM) showed little effect. Intracellular recordings from CA1 pyramidal neurons gave similar dose-dependent results. Low doses increased spontaneous firing rates and caused silent cells to fire. Medium doses both increased and decreased firing rates, whereas high doses depressed firing rates. Large transient depolarizing shifts were seen in some phencyclidine-treated cells at medium and high doses. Phencyclidine effects took 15-30 min to develop and were only partially reversible after a washout of up to 1 h.