5-Hydroxyindoleacetic acid in cerebrospinal fluid reflects serotonergic damage induced by 3,4-methylenedioxymethamphetamine in CNS of non-human primates.
George A. Ricaurte, L. Delanney, S. Wiener, I. Irwin, J. Langston
Brain Research December 6, 1988 DOI: 10.1016/0006-8993(88)90451-9 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Observational animal study Peer reviewed |
|---|---|
| Population | Non-human primates (monkeys) administered toxic doses of MDMA |
| Intervention | MDMA |
| Duration | CSF sampled two weeks after MDMA administration; animals killed the same day |
| Measures | 5-HIAA, homovanillic acid (HVA), 3-methoxy-4-hydroxyphenethyleneglycol (MHPG) |
| Topics | MDMA Serotonin |
| Key findings | Monkeys with 73-94% serotonin and 5-HIAA depletion in brain and 42-45% depletion in spinal cord showed a 60 +/- 7% reduction in CSF 5-HIAA, with no change in HVA or MHPG. The authors conclude that CSF 5-HIAA can detect central serotonergic damage from MDMA in non-human primates and suggest it may be useful for detecting MDMA-induced neuronal damage in humans. |
Abstract
This study examined whether 5-hydroxyindoleacetic acid (5-HIAA) in cerebrospinal fluid (CSF) could be used to detect serotonergic damage induced by (+/-)-3,4-methylenedioxymethamphetamine (MDMA) in the central nervous system (CNS) of non-human primates. Monkeys were administered toxic doses of MDMA; two weeks later, the animals were lightly anesthetized with ether and CSF was obtained by means of cervical puncture. Later that same day, the animals were killed for direct determination of CNS serotonin and 5-HIAA concentrations. Monkeys with 73-94% depletions of serotonin and 5-HIAA in brain and 42-45% depletions of serotonin and 5-HIAA in the spinal cord had a 60 +/- 7% reduction of 5-HIAA in CSF, without any change in homovanillic acid (HVA) or 3-methoxy-4-hydroxyphenethyleneglycol (MHPG). These findings indicate that CSF 5-HIAA can be employed to detect central serotonergic damage produced by MDMA in non-human primates, and suggest that CSF 5-HIAA may be useful for detecting MDMA-induced neuronal damage in humans.