In Vitro Screening of Psychoactive Drugs to Prevent the Abuses
Yakugaku Zasshi December 1, 2008 DOI: 10.1248/yakushi.128.1771 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory assay development and application Peer reviewed |
|---|---|
| Measures | monoamine reuptake and release, [35S]GTPγS binding |
| Topics | MDMA Serotonin |
| Keywords | Tryptamine Pharmacology Designer drug Dopamine Piperazine Phenethylamine Monoamine neurotransmitter Biochemistry Receptor |
| Citations | 4 |
| Key points | The assay system reliably measured monoamine reuptake and release and G-protein activation for various psychoactive drug classes, and some drugs showed central nervous system effects comparable to controlled substances. This allowed designation of prohibited substances under the Tokyo ordinance. |
Abstract
The Tokyo Metropolitan Government in Japan enacted an "Ordinance concerning the abuse prevention of the psychoactive drugs" in April 2006 that prohibited the manufacture, cultivation, sales, possession, use, etc., of these drugs. Therefore, we constructed a reproducible, simple, and small-scale determination method of the psychoactive drugs for the re-uptake and the release of monoamines (dopamine, serotonin and norepinephrine), and the activation of [(35)S]guanosine-5'-O-(3-thio)-triphosphate binding to guanine nucleotide-binding proteins (G proteins). These assays were then applied to study the effects of different kinds (phenethylamine derivatives, tryptamine derivatives, and piperazine derivatives) of non-medically used psychoactive drugs on monoamine re-uptake and release, and G-protein binding. The results suggested that some drugs strongly act on the central nerve system to the same extent as the drugs. This assay system was able to designate psychoactive drugs as prohibited substances in accordance with criteria set forth by the Tokyo Metropolitan government.
Citations in the library
Cites 8
- Amphetamine-type central nervous system stimulants release norepinephrine more potently than they release dopamine and serotonin Synapse 2000
- The effects of non-medically used psychoactive drugs on monoamine neurotransmission in rat brain. European Journal of Pharmacology March 22, 2007
- Hallucinogen-like actions of 5-methoxy-N,N-diisopropyltryptamine in mice and rats. Pharmacology, Biochemistry, and Behavior 2006
- Effects of “Legal X” Piperazine Analogs on Dopamine and Serotonin Release in Rat Brain Annals of the New York Academy of Sciences October 1, 2004
- In Vitro Screening of Psychoactive Drugs by [35S]GTP.GAMMA.S Binding in Rat Brain Membranes Biological and Pharmaceutical Bulletin 2007
and 3 more in the library