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Experimental Study on the Postmortem Redistribution of the Substituted Phenethylamine, 25B‐NBOMe

Kaori Shintani-Ishida, Kanju Saka, Mami Nakamura, Ken-Ichi Yoshida, Hiroshi Ikegaya

Journal of Forensic Sciences March 1, 2018 DOI: 10.1111/1556-4029.13583 (opens in new tab)

Summary

AI-generated from the abstract

A rat model shows that the concentration of the drug 25B-NBOMe in cardiac blood can increase more than tenfold within six hours after death, even when the drug was given a week earlier. The drug accumulates mainly in the lungs. This postmortem redistribution means that toxicological analyses of 25B-NBOMe must account for sampling time and location to avoid misinterpretation.

Study at a glance

Characteristics Observational cohort Peer reviewed
Sample size 36
Population Sprague-Dawley rats
Dose 0.5 mg/kg
Duration 30 min post-injection; postmortem intervals of 0, 3, 6, 9, 15, or 24 h
Key finding Postmortem 25B-NBOMe concentration in cardiac blood increased more than tenfold at six hours after death, and redistribution occurred even when administration was one week prior.

Abstract

Abstract2‐(4‐Bromo‐2,5‐dimethoxyphenyl)‐N‐(2‐methoxybenzyl)ethanamine (25B‐NBOMe) is a substituted phenethylamine, which has become highly prevalent worldwide since 2014. Recently, in an autopsy case involving fatal 25B‐NBOMe intoxication, we found the postmortem increase of 25B‐NBOMe concentration in the cardiac blood approximately 2 days after death. The aim of this study was to investigate the distribution of 25B‐NBOMe and reproduce the postmortem redistribution using a rat model. Sprague‐Dawley rats were killed 30 min after intraperitoneal injection of 25B‐NBOMe (0.5 mg/kg) and left for 0, 3, 6, 9, 15, or 24 h (six rats at each time point). Postmortem 25B‐NBOMe concentrations in the cardiac blood increased by more than 10‐fold at 6‐h postmortem. 25B‐NBOMe accumulated primarily in the lung. Moreover, this postmortem redistribution occurred even in rats that had died 1 week following the 25B‐NBOMe administration. These findings indicate that attention should be paid to sample collection and data interpretation in the toxicological analysis of 25B‐NBOMe.

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