Photolabeling of brain membrane proteins by lysergic acid diethylamide
Life Sciences April 5, 1982 DOI: 10.1016/0024-3205(82)90660-9 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory experiment Peer reviewed |
|---|---|
| Population | Bovine caudate membranes |
| Intervention | ultraviolet light illumination |
| Topics | LSD |
| Citations | 7 |
| Key findings | Photolabeling of bovine caudate membranes with 3H-LSD is not specific for LSD binding sites associated with neurotransmitter receptors and can occur under room lighting, potentially introducing artifacts into receptor binding assays. |
Abstract
3H-Lysergic acid diethylamide (3H-LSD) is irreversibly incorporated into bovine caudate membranes during ultraviolet light illumination. The incorporated radioligand apparently forms a covalent bond with a subpopulation of the membrane proteins. Although the photolabeling pattern differs significantly from the Coomassie blue staining pattern on SDS gels, the photolabeling is apparently not specific for LSD binding sites associated with neurotransmitter receptors. 3H-LSD photolabeling can occur during prolonged exposure of membrane samples to room lighting and thus may introduce artifacts into receptor binding assays.