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Sex-specific increased reactivity of the PVT and prolonged PVT→CeA circuit engagement following psilocin administration.

Devin P. Effinger, J L Hoffman, Sema G. Quadir, Christian S Rollison, Daniel Toedt, Maria Echeveste Sanchez, Margaret W High, Clyde W. Hodge, Melissa A. Herman

Nature Communications April 10, 2026 DOI: 10.1038/s41467-026-71481-1 (opens in new tab)

Study at a glance

AI-extracted from the abstract
Characteristics Experimental study Peer reviewed
Population Rats
Intervention Psilocin
Topics Psilocybin
Citations 1
Key findings Psilocin modulates PVT and PVT→CeA circuitry in a sex-specific manner, engaging these circuits in females but not males.

Abstract

The psychedelic psilocybin has shown therapeutic potential, yet underlying neural mechanisms remain poorly understood. We investigated the impact of psilocin-the active metabolite of psilocybin-on basal activity and reactivity within the paraventricular nucleus of the thalamus (PVT) and PVT projections to central amygdala (CeA) in rats. Psilocin administration increased PVT c-Fos expression and selectively engaged PVT→CeA neurons in females, but not males. Psilocin enhanced PVT reactivity to an aversive air-puff stimulus, with effects primarily driven by passive responders. In PVT→CeA neurons, psilocin prevented time-dependent reductions in stimulus-evoked activity and maintained reactivity across timepoints in females but not males. The sustained engagement of PVT→CeA circuitry was driven by active responders. These findings identify sex-specific modulation of thalamic-limbic circuitry and behavior by psilocin, implicating PVT→CeA circuitry in the neural and behavioral effects of psychedelic compounds, advancing our understanding of how psychedelics modulate emotional brain circuits to further inform potential therapeutic mechanisms.