The bioactivity of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) and its detection in rat urine by capillary electrophoresis combined with an on-line sample concentration technique.
Yu-Chih Chiu, Shiu-Huey Chou, Ju-Tsung Liu, Cheng-Huang Lin
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences November 25, 2004 DOI: 10.1016/j.jchromb.2004.08.021 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Mice and rats |
| Intervention | 2C-T-2 |
| Dose | 20 microg/g of body weight |
| Duration | First- and second-day urine fractions collected after administration |
| Keywords | Analytical chemistry Capillary electrophoresis 2c-t-2 Toxicology Biomonitoring |
| Citations | 18 |
| Key findings | 2C-T-2 reduced NO production in mouse spleen and thymus lymphocytes, and the drug was detectable in rat urine at concentrations down to nanogram per milliliter levels using on-line sample concentration techniques. |
Abstract
The bioactivity of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) on nitric oxide (NO) production and the proliferation of spleen and thymus lymphocytes to mitogen stimulation in mice are reported for the first time. NO production by T and B lymphocytes in spleen and T cells in the thymus of mice decreased after the oral administration of 2C-T-2. This indicates that 2C-T-2 intake may perturb both neural and immune activity since a decrease in NO production is indicative of a weakened defense function. 2C-T-2 (the parent drug) in rat urine samples was detected by means of capillary electrophoresis/UV absorbance combined with an on-line sample concentration technique. When the CZE and MEKC modes were employed, the detection limit was found to be 4.5 and 5.0 microg/mL (at a 92.1% confidence level); whereas when on-line sample concentration methods, including stacking and sweeping-micellar electrokinetic chromatography were used, the detection limits were improved to 19.2 and 9.1 ng/mL, respectively. In an analysis of some actual samples from animal experiments, three male rats were administered 20 microg/g of body weight of 2C-T-2 by intra-peritoneal injection. The first- and second-day urine fractions were collected after the administration, for use in the analysis. As a result, 2.9 microg/mL and 0.25 microg/mL of 2C-T-2, respectively, were detected after ingestion of the doses.