Screening for and validated quantification of phenethylamine-type designer drugs and mescaline in human blood plasma by gas chromatography/mass spectrometry.
Vilma Habrdova, Frank T. Peters, Denis S Theobald, Hans H Maurer
Journal of mass spectrometry : JMS June 1, 2005 DOI: 10.1002/jms.853 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method validation study Peer reviewed |
|---|---|
| Population | Human blood plasma |
| Topics | Mescaline |
| Keywords | Drug detection Substance identification Drug screening Compound analysis Forensic toxicology Drug testing Bioanalysis Drug monitoring Clinical toxicology Poisoning analysis Toxin detection Drug effects Public health monitoring Analytical chemistry Chemical analysis Laboratory methods Gas chromatography-mass spectrometry Gc/ms Scientific techniques Method validation Designer drugs Novel psychoactive substances Nps Synthetic drugs Illicit drugs Phenethylamines Emerging drugs |
| Citations | 61 |
| Key points | A validated GC/MS method was developed for screening and quantification of seven 2C-series drugs and mescaline in human blood plasma, but validation failed for 2C-T-2 and 2C-T-7. |
Abstract
In recent years, several newer designer drugs of the so-called 2C series such as 2C-D, 2C-E, 2C-P, 2C-B, 2C-I, 2C-T-2, and 2C-T-7 have entered the illicit drug market as recreational drugs. Some fatal intoxications involving 2C-T-7 have been reported. Only scarce data have been published about analyses of these substances in human blood and/or plasma. This paper describes a method for screening and simultaneous quantification of the above-mentioned compounds and their analog mescaline in human blood plasma. The analytes were analyzed by gas chromatography/mass spectrometry in the selected-ion monitoring mode, after mixed-mode solid-phase extraction (HCX) and derivatization with heptafluorobutyric anhydride. The method was fully validated according to international guidelines. Validation data for 2C-T-2 and 2C-T-7 were unacceptable. For all other analytes, the method was linear from 5 to 500 microg/L and the data for accuracy (bias) and precision (coefficient of variation) were within the acceptance limits of +/-15% and <15%, respectively (within +/-20% and <20% near the limit of quantification of 5 microg/L).