Facile assembly and fluorescence-based screening method for heterologous expression of biosynthetic pathways in fungi
Metabolic Engineering May 26, 2018 Sandra Hoefgen, Jun Lin, Janis Fricke et al. 113 citations
Heterologous expression of multi-gene biosynthetic pathways in eukaryotic hosts is limited by highly regulated individual monocistrons. Dissimilar to prokaryotes, each eukaryotic gene is strictly controlled by its own regulatory elements, such as promoter and terminator. Consequently, parallel transcription can occur only when a group of genes is synchronously activated. A strategy to...