A method using gas chromatography high-resolution mass spectrometry was developed to detect and measure the designer hallucinogen 5-MeO-DIPT in urine. Two metabolites, 5-OH-DIPT and 5-MeO-IPT, were identified in users' urine. The method accurately quantified 5-MeO-DIPT between 2 and 300 ng/mL, with a detection limit of 1 ng/mL. In drug abusers, urine concentrations ranged from 1 to 2.8 ng/mL. Stability testing showed that 5-MeO-DIPT concentration decreased by 22.8% after 24 hours at 25°C, 33.2% after 5 days at 4°C, and 38.2% after 7 days at 4°C, but was stable for 30 days at -20°C. Freezing urine samples is recommended for accurate analysis.
A new LC-MS/MS method was developed to simultaneously extract LSD, iso-LSD, 2-oxo-3-hydroxy-LSD, and 1-propionyl-LSD from hair samples. The method was applied to hair from 18 suspected LSD users. Segmental hair analysis showed LSD concentrations decreasing from the proximal to the distal end, while 1 P-LSD was not detected in any hair segments. The authors report concentrations of LSD and iso-LSD in human hair from 18 LSD users, but note that interpreting hair analysis results for LSD remains difficult.
A rapid UHPLC-MS/MS method was developed to screen 16 tryptamines in hair samples. Twenty milligrams of hair were pulverized and processed, achieving limits of detection from 0.1 to 20 pg/mg and quantitation from 3 to 50 pg/mg. The method was applied to 191 hair samples from suspected tryptamine users; 77 tested positive for 5-MeO-DiPT, while the other 114 showed none of the targeted tryptamines or metabolites. In 34 samples, 5-MeO-DiPT and its metabolites 5-MeO-NiPT, 5-OH-DiPT, and 4-OH-DiPT were detected together. The method demonstrated good accuracy (91-114%), precision, and recovery (85-115%), making it suitable for forensic analysis of tryptamine use.
A dried urine spot (DUS) method using LC-MS/MS was developed to measure the tryptamine hallucinogen 5-MeO-DIPT, which is unstable in liquid urine. Ten microliters of urine were spotted on a card and extracted with methanol. The method's limit of detection was 0.1 ng/ml and lower limit of quantification was 0.2 ng/ml, with accuracy between 98.2% and 103.9% and precision between 2.7% and 8.5%. 5-MeO-DIPT was more stable in DUS than in urine stored at 25 °C. When applied to urine from known users, concentrations ranged from 0.3 to 2.3 ng/ml, lower than those measured by GC-Orbitrap-MS. The small sample volume and simplicity make this useful for drug screening.