Hydrophillic Interaction Chromatography-tandem Mass Spectrometry Method for Identification and Quantitation of 5-MeO-DIPT and its Metabolites in Rat Urine
Yoon Kim, Un-Yong Kim, Moon Kyo In, Jaeick Lee, Oh‐Seung Kwon, Hye Hyun Yoo
Bulletin of the Korean Chemical Society April 20, 2011 DOI: 10.5012/bkcs.2011.32.4.1158 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory study Peer reviewed |
|---|---|
| Population | Rat urine samples |
| Topics | 5-MeO-DMT |
| Citations | 4 |
| Key points | Identified 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT, and their glucuronide conjugates as metabolites of 5-MeO-DIPT in rat urine, and validated a quantitative HILIC-MS/MS method with linearity over 1-1000 ng/mL and accuracy of 92.2-110.2%. |
Abstract
5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILICMS/MS). Chromatographic separation was achieved on an Atlantis HILIC silica column ( $5{\mu}m$ , $100{\times}2.1\;mm$ ). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 1-1000 ng/mL with linear correlation co-efficients ( $r^2$ ) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.