Repeated Sub-anaesthetic dose of Ketamine Elevates Superoxide Dismutase in Pharmacological Model of Schizophrenia-Like Phenotypes in Mice
Usman Yusuf, Aderibigbe Oladele Adegbuyi, Fehintola Fatai Adewale
Study at a glance
AI-extracted from the abstract| Characteristics | Animal study |
|---|---|
| Sample size | 18 |
| Population | Mice |
| Interventions | Ketamine Risperidone |
| Dose | 20 mg/kg ketamine; 0.5 mg/kg risperidone |
| Duration | 14-day intervention |
| Topics | Esketamine Ketamine |
| Key points | Repeated sub-anaesthetic ketamine increased locomotor activity and cognitive latency and elevated superoxide dismutase activity in mouse brain, with risperidone partially counteracting these changes. |
Abstract
Abstract Objective The study evaluated behavioural phenotypes and the level of Superioxde Dismutase activity in repeated sub-anaesthetic dose of ketamine administered to model schizophrenia in animal study.
Method: Animals were divided into groups (n=6), control group received distilled water (10mL/kg) as vehicle (VEH), Ketamine treated group (KET) received sub-anaesthetic dose of ketamine (20mg/kg) for 14 days consecutively. Animals in risperidone treated group (KET+RISP) were pre-treated with sub-anaesthetic dose of ketamine (20mg/kg) alone for 7 consecutive days, and from day 8-14, risperidone (0.5mg/kg) was administered 1 hour post-ketamine treatment. All treatment were administered intraperitoneally (i.p). Twenty-four (24) hours after the last treatment, Behavioural phenotypes (locomotor activity and cognition) were assessed in locomotor activity cage and elevated maze plus. Thereafter level of superioxde dismutase (SOD) activity was evaluated in homogenized brain tissue of each mouse using spectrophotometric analysis.
Result: KET group showed significant (p<0.05) increase in movement counts and number of rearing events in locomotor activity test, also prolonged latency to enter the open arms in cognitive assessment compared to animals that received distilled water (10mg/kg), and risperidone (0.5mg/kg) treatment. The level of Superioxide Dismutase (SOD) activity was significantly elevated in KET group compare with vehicle control and risperidone treated animals.
Conclusion: Repeated dose of ketamine may pose differential effect on endogenous antioxidant system which may elevate superioxide dismutase activity in ketamine induced schizophrenic rodent as positive control mechanism.
Comparable studies
Other preclinical and animal studies on ketamine, most cited first.
| Study | Year | Design | Participants |
|---|---|---|---|
| mTOR-Dependent Synapse Formation Underlies the Rapid Antidepressant Effects of NMDA Antagonists Rats | 2010 | Observational study | |
| Activation of Glutamatergic Neurotransmission by Ketamine: A Novel Step in the Pathway from NMDA Receptor Blockade to Dopaminergic and Cognitive Disruptions Associated with the Prefrontal Cortex Conscious rats | 1997 | Dose-response study with microdialysis and behavioral testing | |
| NMDAR inhibition-independent antidepressant actions of ketamine metabolites Mice | 2016 | Preclinical study | |
| The dissociative anaesthetics, ketamine and phencyclidine, selectively reduce excitation of central mammalian neurones by N‐methyl‐aspartate Spinal neurons in decerebrate or pentobarbitone-anaesthetized cats and rats | 1983 | Experimental study | |
| R-ketamine: a rapid-onset and sustained antidepressant without psychotomimetic side effects Mice | 2015 | Experimental study |