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Activity-dependent brain-derived neurotrophic factor signaling is required for the antidepressant actions of (2 R ,6 R )-hydroxynorketamine

Kenichi Fukumoto, Manoela V. Fogaça, Rong-Jian Liu, Catharine H. Duman, T. Kato, Xiaoyuan Li, Ronald S. Duman

Proceedings of the National Academy of Sciences December 17, 2018 DOI: 10.1073/pnas.1814709116 (opens in new tab)

Study at a glance

AI-extracted from the abstract
Characteristics Preclinical study Peer reviewed
Population Animal models
Keywords Antidepressant Brain-derived neurotrophic factor Neurotrophic factors Pharmacology Hippocampus Receptor
Citations 191
Key points The antidepressant effects of (2R,6R)-HNK require activity-dependent release of BDNF mediated by stimulation of voltage-dependent Ca2+ channels, leading to TrkB and mTORC1 signaling that increases synaptic function in the medial prefrontal cortex.

Abstract

Significance A metabolite of ketamine, (2 R ,6 R )-hydroxynorketamine [(2 R ,6 R )-HNK], produces rapid and sustained antidepressant effects in animal models but without the side effects of ketamine. Notably, (2 R ,6 R )-HNK does not block the NMDA receptor, the primary target of ketamine. Here, we demonstrate that the antidepressant effects of (2 R ,6 R )-HNK require activity dependent release of BDNF that is mediated by stimulation of voltage-dependent Ca 2+ channels. Importantly, increased BDNF release leads to activation of downstream TrkB and mechanistic target of rapamycin complex 1 signaling that increases synaptic function of pyramidal neurons in the medial prefrontal cortex. Stimulation of BDNF release and increased synaptic function block or reverse the detrimental effects of stress and depression and thereby underlie the antidepressant actions of (2 R ,6 R )-HNK.