N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model.
B. Nemes, K. Peto, N. Németh, Anita Mester, Zsuzsanna Magyar, Souleiman Ghanem, Viktória Sógor, Bence Tánczos, Á. Deák, Márk Kállay, L. Bidiga, E. Frecska
Transplantation Proceedings May 1, 2019 DOI: 10.1016/j.transproceed.2019.04.005 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Randomized controlled trial Peer reviewed |
|---|---|
| Sample size | 26 |
| Population | Anesthetized rats |
| Interventions | N N-dimethyltryptamine |
| Duration | 60 minutes ischemia, 120 minutes reperfusion |
| Topics | 5-MeO-DMT DMT |
| Citations | 21 |
| Key findings | DMT administered before ischemia reduced histologic injury and improved microcirculatory recovery in a rat kidney ischemia-reperfusion model. |
Abstract
Background: Ischemia reperfusion (I/R) injury remains one of the most challenging fields of organ transplantation. It is highly associated with the use of expanded criteria donors that might conclude to delayed graft function or early or late graft failure.
Objective: To investigate the metabolic, microcirculatory parameters, and histologic changes under the effect of N,N-dimethyltryptamine (DMT) in a renal I/R model in rats.
Method: In 26 anesthetized rats both kidneys were exposed. In the control group (n = 6) no other intervention happened. In 20 other animals, the right renal vessels were ligated, and after 60 minutes the right kidney was removed. The left renal vessels were clamped for 60 minutes then released, followed by 120 minutes of reperfusion. In the I/R group (n = 10), there was no additive treatment, while in I/R + DMT group (n = 10) DMT was administered 15 minutes before ischemia. Blood samples were taken, laser Doppler measurement was performed, and both kidneys were evaluated histologically.
Results: Microcirculation (blood flux units [BFU]) diminished in all groups, but remarkably so in the I/R + DMT group. This group compensated better after the 30th minute of reperfusion. The control and I/R + DMT groups had similar BFUs after 120 minutes of reperfusion, but in the I/R group BFU was higher. Tubular necrosis developed in the I/R and I/R + DMT groups too; it was moderated under DMT effect, and severe without. Histologic injuries were less in I/R + DMT Group compared to non-treated animals.
Conclusion: Histologic changes characteristic to I/R injuries were reversible and microcirculation recovered at the end of 120 minutes reperfusion under the administration of DMT. DMT can be used for renoprotection in kidney transplantation.