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Cannabidiol or ketamine for preventing the impact of adolescent early drug initiation on voluntary ethanol consumption in adulthood.

Carles Colom-Rocha, Cristian Bis-Humbert, M Julia García-Fuster

Frontiers in Pharmacology 2024 DOI: 10.3389/fphar.2024.1448170 (opens in new tab)

Study at a glance

AI-extracted from the abstract
Characteristics Preclinical study Peer reviewed
Population Male and female Sprague-Dawley rats
Interventions cannabidiol ketamine
Duration Adolescent treatment from postnatal days 29 to 38; ethanol access for 6 weeks in adulthood
Topics Addiction CBD Esketamine Ketamine
Keywords Addiction risk factors Adolescence Rodent models Sex differences Therapeutical options Addiction-prevention Adolescent-alcohol-exposure Substance-abuse-treatment Animal-research Sex-based-therapeutics
Citations 3
Key points Adolescent ethanol exposure, alone or with cocaine, increases voluntary ethanol consumption in adulthood, and cannabidiol or ketamine (in females) can reduce this consumption.

Abstract

Few studies have previously evaluated the long-term impact of initiating the combined use of alcohol and cocaine early-in-life during adolescence. Our preclinical study characterized changes in affective-like behavior and/or voluntary ethanol consumption emerging later on in adulthood induced by a prior adolescent drug exposure, as well as tested therapeutical interventions (i.e., cannabidiol or ketamine) to prevent the observed effects. We performed three independent studies with male and female Sprague-Dawley rats, treated in adolescence (postnatal days, PND 29-38) with non-contingent paradigms of ethanol, cocaine, their combination or vehicle. Later on, adult rats were (1) scored for their affective-like state (forced-swim, elevated-plus maze, novelty-suppressed feeding, sucrose preference), (2) allowed to freely drink ethanol for 6 weeks (two-bottle choice), or (3) treated with cannabidiol or ketamine before given access to ethanol in adulthood. No signs of increased negative affect were observed in adulthood following the adolescent treatments. However, adolescent ethanol exposure was a risk-factor for later developing an increased voluntary ethanol consumption in adulthood, both for male and female rats. This risk was similar when ethanol was combined with adolescent cocaine exposure, since cocaine alone showed no effects on later ethanol intake. Finally, rats exposed to adolescent ethanol and pretreated in adulthood with cannabidiol (and/or ketamine, but just for females) reduced their ethanol voluntary consumption. Our data provided two therapeutical options capable of preventing the impact of an early drug initiation during adolescence by decreasing voluntary ethanol consumption in adult rats.

Comparable studies

Other preclinical and animal studies on ketamine for addiction, most cited first.

Study Year Design Participants
R-(-)-ketamine modifies behavioral effects of morphine predicting efficacy as a novel therapy for opioid use disorder. Morphine-dependent rats and mice 2020 Preclinical study
The effects of (2R,6R)-hydroxynorketamine on oxycodone withdrawal and reinstatement. Male and female oxycodone-dependent mice 2023 Preclinical study
Characterizing the therapeutical use of ketamine for adolescent rats of both sexes: Antidepressant-like efficacy and safety profile. Adolescent rats of both sexes, including naïve and early-life stressed (maternal... 2025 Preclinical study
Exploring ketamine's reinforcement, cue-induced reinstatement, and nucleus accumbens cFos activation in male and female long evans rats. Long Evans rats 2024 Preclinical experimental study
Brain acid sphingomyelinase controls addiction-related behaviours in a sex-specific way. Male and female mice with forebrain ASM overexpression 2025 Experimental study

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