De novo sequencing and analysis of Lophophora williamsii transcriptome, and searching for putative genes involved in mescaline biosynthesis
Enrique Ibarra‐laclette, Flor Zamudio Hernández, Claudia Pérez-torres, Victor A. Albert, Enrique Ramı́rez-chávez, Jorge Molina‐torres, Araceli Fernández-cortés, Carlos Calderón‐vázquez, José Luís Olivares-Romero, Alfredo Herrera‐estrella, Luís Herrera‐estrella
BMC Genomics September 1, 2015 DOI: 10.1186/s12864-015-1821-9 (opens in new tab) via OpenAlex
Summary
AI-generated from the abstractHigh-throughput transcriptome sequencing (RNA-seq) identified candidate genes—tyrosine/DOPA decarboxylase, hydroxylases, and O-methyltransferases—likely involved in mescaline biosynthesis in Lophophora williamsii. This provides a theoretical basis for designing bioassays to confirm these genes' roles in mescaline production.
Study at a glance
| Characteristics | Transcriptome sequencing analysis Peer reviewed |
|---|---|
| Population | Lophophora williamsii |
| Topics | Mescaline |
| Keywords | Kegg Transcriptome Sequence assembly Genetics |
| Citations | 26 |
| Key finding | Candidate genes including tyrosine/DOPA decarboxylase, hydroxylases, and O-methyltransferases were identified as potentially involved in mescaline biosynthesis. |
Abstract
High throughput transcriptome sequencing (RNA-seq) analysis allowed us to efficiently identify candidate genes involved in mescaline biosynthetic pathway in L. williamsii; these included tyrosine/DOPA decarboxylase, hydroxylases, and O-methyltransferases. This study sets the theoretical foundation for bioassay design directed at confirming the participation of these genes in mescaline production.